microarray suite v 5.0 software  (Thermo Fisher)

Journal: Frontiers in Cell and Developmental Biology

Article Title: Time and age dependent regulation of neuroinflammation in a rat model of mesial temporal lobe epilepsy: Correlation with human data

doi: 10.3389/fcell.2022.969364

Figure Lengend Snippet: Time- and age-dependent expression of inflammatory genes after KA-SE: qRT-PCR validation and human data correlation. (A) Fold change in expression in select inflammatory genes: comparison between the microarray and qRT-PCR results in rats at P30. (B) Fold change in expression in select inflammatory genes: comparison between the microarray and quantitative RT-PCR results in human. T, temporal lobe; H, hippocampus. (C) Inflammatory genes comprised a significant proportion of persistently upregulated genes at 240 h after KA-SE in P30. Red dots represent inflammatory genes. Green dots represent non-inflammatory genes. (D) Summary graph of the time course of fold change in inflammatory genes. Note the inflammatory genes remain significantly elevated at 240 h only in P30 rats.

Article Snippet: Affymetrix Microarray Suite (v. 5.0) was used for probe-level analysis.

Techniques: Expressing, Quantitative RT-PCR, Microarray

Journal: Frontiers in Cell and Developmental Biology

Article Title: Time and age dependent regulation of neuroinflammation in a rat model of mesial temporal lobe epilepsy: Correlation with human data

doi: 10.3389/fcell.2022.969364

Figure Lengend Snippet: Verification of MAPK pathway genes found in animal experiments. (A) qRT-PCR verification of microarray data of some differentially expressed MAPK pathway genes 24 h after KA-SE in P30 animals. (B) Hippocampal sections stained for Hsp70. High magnification (×20) view of CA1 subfield 24 h after KA-SE at P15 and P30 [ (i) P15 PBS; (ii) P15 KA; (iii) P30 PBS; (iv) P30 KA]. Notice robust Hsp 70 immunoreactivity in P15, undetectable in control P30, and induction by KA-SE at 24 h in P30. (C) Hippocampal sections stained for CD74 24 h after KA-SE [ (i) P15 PBS; (ii) P15 KA; (iii) P30 PBS; (iv) P30 KA]. (D) Hippocampal sections stained for CD74 240 h after KA-SE [ (i) P15 PBS; (ii) P15 KA; (iii) P30 PBS; (iv) P30 KA]. CD74 immunoreactive cells are most notable in P30 240 h after KA-SE. (E) MAPK pathway genes found in microarray analysis of hippocampus from patients with MTLE superimposed on MAPK pathway genes found in rats after KA-SE. Human genes: open square = single-pathway associated gene; star = multiple-pathway associated gene. Rat genes: filled circle = single-pathway associated gene; filled triangle = multiple-pathway associated gene.

Article Snippet: Affymetrix Microarray Suite (v. 5.0) was used for probe-level analysis.

Techniques: Quantitative RT-PCR, Microarray, Staining